Journal: Nutrients

Article Title: Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

doi: 10.3390/nu13093209

Figure Lengend Snippet: Effect of LE on IL-4, IgE, and histamine levels in the serum of NC/Nga mice. The levels of ( A ) IL-4, ( B ) IgE, and ( C ) histamine in the serum. Serum was collected on the last day of the experiment and measured using ELISA. The results were expressed as means ± SD ( n = 6). ### p < 0.001 vs. normal (DNCB untreated group), *** p < 0.001 vs. DNCB (negative control; DNCB treated group), PD (positive control; prednisolone 3 mg/kg) treatment group, LE (LE 50, 100 or 200 mg/kg) treatment group, and † p < 0.05, ††† p < 0.001 vs. PD (positive control; prednisolone 3 mg/kg) treatment group.

Article Snippet: The levels of IgE, histamine, IgG1, and IgG2a in the serum were measured using a mouse enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (IgE, Shibayagi, Gunma, Japan; IgG1 and IgG2a, Enzo Life Sciences, Farmingdale, NY, USA; histamine, Elabscience, Huston, ID, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Positive Control

Journal: Nutrients

Article Title: Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

doi: 10.3390/nu13093209

Figure Lengend Snippet: Effect of LE on the levels of IgG1, IgG2a, and the IgG1/IgG2a ratio in the serum of NC/Nga mice. The levels of ( A ) IgG1, ( B ) IgG2a, and ( C ) IgG1/IgG2a ratio (%) in the serum. Serum was collected on the last day of the experiment and measured using ELISA. The results were expressed as means ± SD ( n = 6 ). ### p < 0.001 vs. normal (DNCB untreated group), * p < 0.05, *** p < 0.001 vs. DNCB (negative control; DNCB treated group), PD (positive control; prednisolone 3 mg/kg) treatment group, LE (LE 50, 100, or 200 mg/kg) treatment group, and †† p < 0.01, ††† p < 0.001 vs. PD (positive control; prednisolone 3 mg/kg) treatment group.

Article Snippet: The levels of IgE, histamine, IgG1, and IgG2a in the serum were measured using a mouse enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (IgE, Shibayagi, Gunma, Japan; IgG1 and IgG2a, Enzo Life Sciences, Farmingdale, NY, USA; histamine, Elabscience, Huston, ID, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Positive Control

Journal: Nutrients

Article Title: Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

doi: 10.3390/nu13093209

Figure Lengend Snippet: Effect of LE on AD-related cytokines and chemokines in the serum of NC/Nga mice. ( A – H ) The levels of ( A ) IL-1β, ( B ) IL-6 ( C ) TNF-α, ( D ) TSLP, ( E ) TARC ( F ) MDC, ( G ) RANTES, and ( H ) MCP-1 in the serum. Serum was collected on the last day of the experiment and measured using ELISA. The results were expressed as means ± SD ( n = 6). ### p < 0.001 vs. normal (DNCB untreated group), *** p < 0.001 vs. DNCB (negative control; DNCB treated group), PD (positive control; prednisolone 3 mg/kg) treatment group, LE (LE 50, 100 or 200 mg/kg) treatment group, and † p < 0.05, †† p < 0.01, and ††† p < 0.001 vs. PD (positive control; prednisolone 3 mg/kg) treatment group.

Article Snippet: The levels of IgE, histamine, IgG1, and IgG2a in the serum were measured using a mouse enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (IgE, Shibayagi, Gunma, Japan; IgG1 and IgG2a, Enzo Life Sciences, Farmingdale, NY, USA; histamine, Elabscience, Huston, ID, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Positive Control

Journal: Nutrients

Article Title: Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

doi: 10.3390/nu13093209

Figure Lengend Snippet: Effect of LE on AD-related cytokine and chemokine secretion in NC/Nga mouse splenocytes. ( A ) The levels of ( A-a ) IL-4, ( A-b ) IL-5, ( A-c ) IL-13, and ( A-d ) IL-31 in the supernatant of splenocytes. ( B ) The levels of ( B-a ) IL-25, ( B-b ) IL-33, and ( B-c ) TSLP in the supernatant of splenocytes. ( C ) The levels of ( C-a ) MCP-1 in the supernatant of splenocytes. ( D ) The levels of ( D-a ) IL-1β, ( D-b ) IL-6, and ( D-c ) TNF-α in the supernatant of splenocytes. ( E ) The levels of ( E-a ) IL-17 and ( E-b ) IL-22 in the supernatant of splenocytes. ( F ) The levels of Th1-mediated cytokines ( F-a ) IL-12 and ( F-b ) IFN-γ. Splenocytes from NC/Nga mice were stimulated with Con-A for 72 h, and then the supernatant was measured using ELISA. Cytokines and chemokines were normalized to the protein concentration of the lysate. The results were expressed as means ± SD ( n = 6). ## p < 0.01, ### p < 0.001 vs. normal (DNCB untreated group), ** p < 0.01, *** p < 0.001 vs. DNCB (negative control; DNCB treated group), PD (positive control; prednisolone 3 mg/kg) treatment group, LE (LE 50, 100 or 200 mg/kg) treatment group, and † p < 0.05, †† p < 0.01, and ††† p < 0.001 vs. PD (positive control; prednisolone 3 mg/kg) treatment group.

Article Snippet: The levels of IgE, histamine, IgG1, and IgG2a in the serum were measured using a mouse enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (IgE, Shibayagi, Gunma, Japan; IgG1 and IgG2a, Enzo Life Sciences, Farmingdale, NY, USA; histamine, Elabscience, Huston, ID, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration, Negative Control, Positive Control

Journal: Polymers

Article Title: Compound Microalgae-Type Biofunctional Hydrogel for Wound Repair during Full-Thickness Skin Injuries

doi: 10.3390/polym16050692

Figure Lengend Snippet: Antioxidant activity and anti-inflammatory properties of hydrogels. ( a – f ) The antioxidant capacity of different concentrations of HQCS-SP to scavenge ABTS· + , DPPH, and ·OH. ( g ) Confocal fluorescence images of NIH-3T3 cells stained with DCFH-DA and Hoechst 33342 after different treatments, Scale bar: 100 µm; ( h ) Effects of DCFH-DA fluorescence intensity. ( i ) Average fluorescence intensity of DCF in cells. ( j , k ) The expression of pro-inflammatory factors (IL-6, TNF- α ) in LPS-activated RAW 264.7 cells with different treatments. (* p < 0.05, ** p < 0.01, *** p < 0.001, different low case letters above columns indicate statistical differences at p < 0.05).

Article Snippet: Mouse IL-6 and TNF- α enzyme-linked immunosorbent assay (ELISA) kits were purchased from Signalway Antibody LLC (College Park, MD, USA) ( https://www.sabbiotech.com/ , accessed on 21 February 2023).

Techniques: Antioxidant Activity Assay, Fluorescence, Staining, Expressing

Journal: Polymers

Article Title: Compound Microalgae-Type Biofunctional Hydrogel for Wound Repair during Full-Thickness Skin Injuries

doi: 10.3390/polym16050692

Figure Lengend Snippet: A full-layer mouse skin defect model that assesses wound healing. ( a ) Representative photograph of wound healing under different treatment modalities. Scale bar: 2 mm. ( b ) Quantification of wound closure rate. ( c ) Graph of the evolution of wound area by the group over time. ( d ) IL-6 and ( e ) IL-10 content in the wound sites of various groups on different days by ELISA assay. (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Mouse IL-6 and TNF- α enzyme-linked immunosorbent assay (ELISA) kits were purchased from Signalway Antibody LLC (College Park, MD, USA) ( https://www.sabbiotech.com/ , accessed on 21 February 2023).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of pharmaceutical sciences

Article Title: VAGINAL SUPPOSITORIES CONTAINING SHetA2 TO TREAT CERVICAL DYSPLASIA: PHARMACOKINETICS OF DAILY DOSES AND PRELIMINARY SAFETY PROFILE

doi: 10.1016/j.xphs.2020.02.016

Figure Lengend Snippet: Cyclin D1 expression level in cervix tissue in mice after vaginal administration of a single or multiple doses of SHetA2 (30 mg/kg, every 24 for five days) compared to that of untreated controls and those receiving placebo suppositories. (A) Multiple dose treatment, (B) comparison of multiple and single dose treatments. Cyclin D1 expression in placebo and SHetA2-treated groups was normalized with the expression level of cyclin D in the untreated controls (* indicates p value less than 0.05, ns= not significant p values were derived using two-way ANOVA Tukey’s post-test, n=5)

Article Snippet: Mouse cyclin D1 Enzyme Linked Immunosorbent Assay (ELISA) kits were purchased from Cedarlane lab (NC).

Techniques: Expressing, Comparison, Derivative Assay